Laser Induced Fluorescence: Application to Spectroscopy and New Microscopy Imaging Methods
Abstract
Laser induced fluorescence is one of the light using techniques which allows the highest sensitivity for atoms and molecules detection, up to the single atom or single molecule level. This field is much too large for an extensive review; therefore we have chosen to focus on two main points: 1- the observation of laser stimulated fluorescence in phthalocyanine and porphyrin like molecules in rare gas and nitrogen matrices at low temperatures. 2- the presentation of laser induced fluorescence techniques suitable for achieving ultra-high spatial resolution imaging, below the diffraction limit of conventional microscopy, thanks to highly fluorescent molecules to be used as biological markers.
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