Optimizing the Dilution Protocol in Continuous Cell Cultures
AbstractDue to its potential impact on the biotech industry, continuous cell cultures have attracted great interest in recent years. However, this type of culture remains expensive and, in some aspects, ineffective. They are often optimized based on the empirical experience of the personnel involved in their development and maintenance, but this is time-consuming and leads to material and nutrients waste that could be reduced with a deeper understanding of the process. We propose an optimization method for continuous cell culture applying techniques from the theory of Optimum Control. As a proof of concept, we apply this method in a simple metabolic network that consumes glucose and secretes lactate. We predict the most appropriate behavior for the dilution rate that maximizes the cell density achieved in the steady state of the system.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
This work is licensed under the Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) license.